Methods and preliminary findings

Sanding was used to produce fragments from automobile coatings with n-CuPc as well as from a reference coating with the same matrix composition but without n-CuPc. Size distribution and agglomeration of these fragments in Rosewell Park Memorial Institute (RPMI) cell culture medium was studied before conducting a battery of cytotoxicity experiments. Cell viability and reactive oxygen species (ROS) production were conducted and particle localization within cells was analyzed.